Zur Seitenansicht

Titelaufnahme

Titel
Osteopontin is a key player for local adipose tissue macrophage proliferation in obesity
Verfasser / VerfasserinStulnig, Thomas Michael ; Zeyda, Maximilian ; Staffler, Gunther ; Gruen, Nicole G. ; Wanko, Bettina ; Moreno-Viedma, Veronica ; Zeyda, Karina ; Tardelli, Matteo
BeteiligtStulnig, Thomas Michael [Corresponding author]
Enthalten in
Molecular Metabolism, Amsterdam, 2016, 5 (2016), 11, S. 1131-1137
ErschienenAmsterdam : Elsevier, 2016
MaterialOnline-Ressource
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
Schlagwörter (EN)OPN / Obesity / Inflammation / Adipose tissue / Adipose tissue macrophage
ISSN2212-8778
URNurn:nbn:at:at-ubmuw:3-63 
DOI10.1016/j.molmet.2016.09.003 
Zugriffsbeschränkung
 Das Dokument ist frei verfügbar
Links
Nachweis
Dateien
Klassifikation
Abstract

Objective

Recent findings point towards an important role of local macrophage proliferation also in obesity-induced adipose tissue inflammation that underlies insulin resistance and type 2 diabetes. Osteopontin (OPN) is an inflammatory cytokine highly upregulated in adipose tissue (AT) of obese and has repeatedly been shown to be functionally involved in adipose-tissue inflammation and metabolic sequelae. In the present work, we aimed at unveiling both the role of OPN in human monocyte and macrophage proliferation as well as the impact of OPN deficiency on local macrophage proliferation in a mouse model for diet-induced obesity.

Methods

The impact of recombinant OPN on viability, apoptosis, and proliferation was analyzed in human peripheral blood monocytes and derived macrophages. Wild type (WT) and OPN knockout mice (SPP1KO) were compared with respect to in vivo adipose tissue macrophage and in vitro bone marrow-derived macrophage (BMDM) proliferation.

Results

OPN not only enhanced survival and decreased apoptosis of human monocytes but also induced proliferation similar to macrophage colony stimulating factor (M-CSF). Even in fully differentiated monocyte-derived macrophages, OPN induced a proliferative response. Moreover, proliferation of adipose tissue macrophages in obese mice was detectable in WT but virtually absent in SPP1KO. In BMDM, OPN also induced proliferation while OPN as well as M-CSF-induced proliferation was similar in WT and SPP1KO.

Conclusions

These data confirm that monocytes and macrophages not only are responsive to OPN and migrate to sites of inflammation but also they survive and proliferate more in the presence of OPN, a mechanism also strongly confirmed in vivo. Therefore, secreted OPN appears to be an essential player in AT inflammation, not only by driving monocyte chemotaxis and macrophage differentiation but also by facilitating local proliferation of macrophages.

Statistik
Das PDF-Dokument wurde 11 mal heruntergeladen.
Lizenz-/Rechtehinweis
Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitung 4.0 International Lizenz